Matthew J. Gage
Department of Chemistry and Biochemistry
P.O. Box 5698
Northern Arizona University
Flagstaff AZ, 86011-5698
(928) 523-6394 matthew.gage@nau.edu
2001 Ph.D. in Biochemistry and Molecular Biology Purdue University
Dissertation title: ÒInteraction of the Virally Encoded Fungal Toxin KP4 with Calcium ChannelsÓ
Advisor: Prof. Thomas J. Smith
1996 B.S. in Chemistry with Honors University of Wyoming
B.S. in Molecular Biology with Honors University of Wyoming
2005 – Present Assistant Professor
Northern Arizona University, Department of Chemistry and Biochemistry
2001 – 2005 Postdoctoral Research Associate
University of Delaware, Department of Chemical Engineering
Advised by Professor Anne Skaja Robinson
1996 – 2001 Research Assistant
Purdue University, Department of Biology
Advised by Professor Thomas J. Smith
1995-1996 Undergraduate Research Assistant,
University of Wyoming, Department of Chemistry
Advisor – Dr. Dean Roddick
1994-1995 Undergraduate Research Assistant,
University of Wyoming, Department of Chemistry
Advisor – Dr. Edward Clennan
1999 – 2001 NIH Biophysics Training Grant, Purdue University
1996 Honor Book, Department of Molecular Biology, University of Wyoming
1995 NSF REU Fellow, Department of Chemistry, University of Wyoming
1994 – 1996 Outstanding Student, Department of Chemistry, University of Wyoming
1994 – 1996 DeanÕs List, University of Wyoming
1. Heintze, E., C. Aguilera, M. Davis, A. Fricker, M.J. Gage. ÒExposure to uranium complexes results in upregulation of p53 mediated pathwaysÓ In review by J. Inorg Biochem..
2. Molloy, R.G., W. K. Ma, A. C. Allen, K. Greenwood, L. Bryan, R. Sacora, L. Williams, M. J. Gage. ÒAquifex aeolicus FlgM protein does not exhibit the disordered character of the Salmonella Typhimurium FlgM protein.Ó Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics doi:10.1016/j.bbapap.2010.03.002
3. Webber, T. M., A. C. Allen, W.K. Ma, R.G. Molloy, C. N. Kettelkamp, C.A. Dow, M.J. Gage. (2009). "Conformational detection of p53's oligomeric state by FlAsH Fluorescence." Biochem Biophys Res Commun 384(1): 66-70.
4. Webber, T., S. Gurung, J. Saul, T. Baker, M. Spatara, M. Freyer, A.S. Robinson, M.J. Gage. (2009). "The C-terminus of the P22 tailspike protein acts as an independent oligomerization domain for monomeric proteins." Biochem J 419(3): 595-602.
5. Gage, M.J., J.L. Zak, and A.S. Robinson, 2005. Three Amino Acids that are Critical to Formation and Stability of the P22 Tailspike Trimer. Protein Science, 14(9): 2333-2343
7. Lefebvre, B.L., N.K. Comolli, M.J. Gage, A.S. Robinson, 2004. Pressure Dissociation Studies Provide Insight Into Oligomerization Competence of Temperature-Sensitive Mutants of P22 Tailspike, Protein Science. 13(6): 1538-46.
8. Lefebvre, B.L., M.J. Gage, A.S. Robinson, 2004. Maximizing Recovery of Native Protein from Aggregates by Optimizing Pressure Treatment, Biotechnology Progress. 20(2): 623-9.
9. Gage, M.J., A. S. Robinson, 2003. C-Terminal Hydrophobic Interactions Play a Critical Role in Oligomeric Assembly of the P22 Tailspike Trimer Protein Science. 12(12): 2732-47.
10. Gage, M.J., S. G. Rane, G. H. Hockerman, T. J. Smith, 2002. The Virally Encoded Fungal Toxin KP4 Specifically Blocks L-Type Voltage Gated Calcium Channels. Molecular Pharmacology 61 (4) 936-944.
11. Gage, M.J., J. Bruenn, M. Fischer, D. Sanders, T. J. Smith, 2001. KP4 Fungal Toxin Inhibits Growth in Ustilago maydis by Blocking Calcium Uptake. Molecular Microbiology. 41(4):775-785.
Provisional Patents
1. Allen, A..C., Gilmore, S., Browder, C.B., Gage, M..J. U.S. Provisional Application # 61/305,816. Filed 2/10
2. Holguin, T.R., Browder, C.B., Gage, M..J. U.S. Provisional Application # 61/305,807. Filed 2/10
1. ÒAquifex aeolicus FlgM protein exhibits a temperature dependent disordered natureÓ Poster, Rhett G. Molloy, Wai Kit Ma, Andrew C. Allen, Kevin Greenwood, Lynn Bryan, Rebecca Sacora, LaBrittney Williams, Matthew J. Gage, Feb 2010, Biophysical Society Meeting, San Francisco, CA
2. ÒAssessing the degree of disorder in homologous FlgM proteinsÓ, Poster, Wai Kit Ma, Matthew J. Gage, Feb 2010, Biophysical Society Meeting, San Francisco, CA
3. ÒApplying Thermodynamics to Fragment-Based Drug DevelopmentÓ, Poster, Tomas R. Holguin, Michael T. Pass, Cindy C. Browder, Matthew J. Gage, Feb 2010, Biophysical Society Meeting, San Francisco, CA
4. ÒExposure Of Human Colon Tumor Cells To Uranyl Acetate Does Not Elicit A p53-mediated Response,Ó Poster, Camille Aguilera, Ellie Heintze and Matthew J. Gage, Nov 2008, Annual Biomedical Research Conference for Minority Students, Orlando
5. ÒAnalysis of uranium exposure on the tumor suppressing protein p53,Ó Poster, Ellie Heintze and Matthew J. Gage, April 2008, American Association of Cancer Research annual meeting, San Diego
6. ÒExploring The Utility Of FlAsH To Measure Protein-Protein Association Events In Real TimeÓ Poster, Tawnya Webber and Matthew J. Gage, Feb. 2008, Biophysical Society Meeting, Long Beach, CA
7. ÒProbing Protein Structure Using the Fluorescent Probe FlAsHÓ Seminar, October, 2007
8. ÒDevelopment of a novel technique to monitor protein-protein interactions in vivoÓ Poster, Tawnya Webber, Eric Johansen, Matthew J. Gage , July 2007, Protein Society Meeting, Boston MA.
9. Characterizing morphologies of the aggregated p53 using TEM, Poster, Ashley Steiner and Matthew J. Gage July 2007, Protein Society Meeting, Boston MA.
10. ÒAggregation Properties of the Tumor Suppressor Protein p53,Ó Poster, Jilleen Jones and Matthew J. Gage July 2007, Protein Society Meeting, Boston MA.
11. ÒUsing Fluorescence to Probe the Structural State of Intrinsically Disordered ProteinsÓ Poster, LaBrittney R. Williams, Trenton Baker and Matthew J. Gage, July 2007, Protein Society Meeting, Boston MA.
12. ÒDetermining Ideal Buffer Conditions for the Fluorescent Probe FlAsH,Ó Poster, Eric Johansen and Matthew J. Gage, CENS Annual Celebration of Undergraduate Research Day, 2007
13. ÒThe C-terminus of the P22 Tailspike Protein Induces Oligomerization of the Maltose-Binding Protein,Ó Poster, Sarsati Gurung, Justin Saul, Trenton Baker, Anne Skaja Robinson, Matthew Gage, CENS Annual Celebration of Undergraduate Research Day, 2007
14. ÒAggregation properties of the tumor suppressor protein p53, Poster, Ashley Steiner, Jilleen Jones, and Matthew J. Gage, CENS Annual Celebration of Undergraduate Research Day, 2007
15. ÒProbing for Flexible Regions of the p53 DNA Binding Domain Through Mass Spectroscopy,Ó Poster, Casey Goodyear, Matthew J. Gage, CENS Annual Celebration of Undergraduate Research Day, 2007
16. ÒUsing Fluorescence to Probe the Structural State of IDPsÓ Poster, LaBrittney R. Williams, Trenton Baker and Dr. Matthew J. Gage, April 2007, CENS Annual Celebration of Undergraduate Research Day, 2007
17. ÒAggregation properties of the tumor suppressor protein p53, Poster, Ashley Steiner, Jilleen Jones, and Matthew J. Gage, ANAS meeting, 2007
18. ÒProbing for Flexible Regions of the p53 DNA Binding Domain Through Mass Spectroscopy,Ó Poster, Casey Goodyear, Matthew J. Gage, ANAS meeting, 2007
19. ÒThe C-terminus of the P22 Tailspike Protein Induces Oligomerization of the Maltose-Binding Protein,Ó Poster, Sarsati Gurung, Justin Saul, Trenton Baker, Anne Skaja Robinson, Matthew Gage, ANAS meeting 2007
20. ÒDevelopment of a novel technique to monitor protein-protein interactions in vivo,Ó Seminar, Tawnya Webber, Matthew Gage, ANAS meeting, 2007
21. ÒUsing Fluorescence to Probe the Structural State of p27Kip1, an Intrinsically Disordered protein,Ó Seminar, Trenton Baker, LaBrittney Williams, Matthew J. Gage, ANAS meeting, 2007
22. ÒToxicity of Uranyl Acetate on p53 in ALTR Cell Lines,Ó Seminar, Ellie Heintze, Matthew J. Gage, ANAS meeting 2007
23. ÒDevelopment of a method to monitor protein-protein interactions using the fluorescent probe FlAsHÓ Poster, Tawnya Webber, Justin Saul, Eric Johansen, Matthew J. Gage, June 2007, Gordon Research Conference, Holderness, NH.
24. ÒUsing LumioTM Binding to Measure Domain-Domain InteractionsÓ Poster, Tawnya Webber, Eric Johansen, Matthew J. Gage, March 2007, Biophysical Society Meeting, Baltimore, MD.
25. ÒCharacterization of the C-terminus of P22 Tailspike Protein,Ó Poster, Sarsati Gurung and Matthew J. Gage, CENS Annual Celebration of Undergraduate Research Day, 2006
26. ÒProbing for Induced Structure in Intrinsically Disordered Proteins.Ó Poster, Trenton L. Baker and Matthew J. Gage, CENS Annual Celebration of Undergraduate Research Day, 2006
27. ÒIntrinsically Disordered Proteins — Are they truly disordered in the cell?Ó Poster, Trenton Baker and Matthew Gage, ANAS 2006
28. ÒFolding of the P22 Tailspike Protein: Applying the Lessons to p53.Ó Seminar, September 2005, Arizona Cancer Center, University of Arizona, Toucan, AZ.
29. ÒIt All Comes Down to Positioning: Three Amino Acids that are Critical to Formation and Stability of the P22 Tailspike Trimer.Ó Seminar, December 2004, Northern Arizona University, Flagstaff, AZ.
30. ÒIt All Comes Down to Positioning: Three Amino Acids that are Critical to Formation and Stability of the P22 Tailspike Trimer.Ó Seminar, November 2004, Trinity University, San Antonio, TX.
31. ÒIt All Comes Down to Positioning: Three Amino Acids that are Critical to Formation and Stability of the P22 Tailspike Trimer.Ó Poster, August 2004, Protein Society Meeting, San Diego, CA
32. ÒExperimental Development of a Model for b-Helical Aggregate GrowthÓ Poster, June 2004, FASEB Summer Research Conference
33. ÒResidues 549, 563 and 572 play a critical role in stability of the P22 tailspike protein,Ó Poster, February 2004, Biophysical Society Meeting, Baltimore, MD
34. ÒHydrophobic Interactions Play a Critical Role in Assembly of the P22 Tailspike Trimer,Ó Seminar, March 2003, Biophysical Society Meeting, San Antonio, TX
35. ÒInteraction of the Virally Encoded Fungal Toxin KP4 with Calcium Channels,Ó Dissertation Seminar, November 2001, Purdue University, West Lafayette, IN
36. ÒThe Interaction of KP4 with Fungal Calcium Channels,Ó Seminar, September 2001, Structural Biology Group, Purdue University, West Lafayette, IN
37. ÒAn Exploration of the Mechanism of KP4,Ó Seminar, June 2001, Thermal Biology Institute, Montana State University, Bozeman, MT
38. ÒInsights into the Mechanism of a Virally Encoded Fungal Toxin,Ó Seminar, December 1999, 5th Annual Biophysics Symposium, Purdue University, West Lafayette, IN
39. ÒInsights into the Mechanism of a Virally Encoded Fungal Toxin,Ó Seminar, November 1999, WISPUR meeting, Argonne National Laboratory, Argonne, IL
2007 - present Professor, Fundamental Biochemistry (2007 – 2009)
Professor, Biochemistry Laboratory (2007 – present)
Professor, Biochemistry (2009 – present)
Professor, Protein structure and Function (Fall 2009)
Northern Arizona University, Department of Chemistry and Biochemistry
2004-2005 Instructor, Introductory Biochemistry
University of Delaware, Department of Chemistry
1996-2005 Research Supervisor
University of Delaware, Department of Chemical Engineering
Purdue University, Department of Biology
1999 Teaching Assistant, Immunology
Purdue University, Department of Biology
1994-1996 Teaching Assistant, General Chemistry
University of Wyoming, Department of Chemistry
1995 Chemistry tutor,
University of Wyoming, Department of Chemistry
Masters: Tawnya Webber (Graduated 2008), Ellie Heintze (Graduated 2008), Camille Aguilera, Malia Davis, Michael Pass, Andy Allen, Rhett Molloy
Undergraduate: Sarsati Gurung, Trenton Baker, Justin Saul, Kristin Heath, Karim Hackler, Eric Fanucci, Casey Goodyear, Ashley Steiner, Jilleen Jones, LaBrittney Williams, Eric Johansen, Catherine Stepnitz, Caitlin Dow, Holly Tiffany, Tomas Holguin (current), Lynn Bryan (current), Amber Enriquez (current), Avery Fricker (current), Wai Kit Ma (current), Kevin Greenwood (current), Allison Grace (current), Jason McCabe (current), Shauna Cooney (current)
1. Defense Threat Reduction Agency (2010 – 2012) $940,118
Development of Inhibitors of the Methionine and Tryptophan Biosynthetic Pathways
2. Pilot Project (NACP, U54) (2009-2011) $143,285
Allostery and Cellular Distribution of the Nitric Oxide Receptor, Soluble Guanylyl Cyclase
3. Growing Biotech Initiative (2008-9) $25,000
Identification of novel targets for antibiotic development
4. Intramural Grant Program (2008-9) $13,000
Identification of novel antibiotics
5. Science Foundation Arizona Competitive Advantage Award (2007) $101,727
Developing Techniques to Measure the Structural State of Intrinsically Disordered Proteins in vivo
6. Growing Biotech Initiative (2007) $25,000
Developing a method to measure subunit-subunit interactions inside of a cell
7. Growing Biotech Initiative (2007) $75,000
Enhancement of Translational Research and Workforce Development at NAU: Acquisition of a Dynamic Light Scattering Apparatus
8. Intramural Grant Program (2007) $9,600
Investigating the link between aggregation of p53 and tumor formation
9. Pilot Project (NACRP, U54) (2006) $60,404
Deciphering the folding pathway of the p53 tumor suppressor protein
10. Growing Biotech Initiative (2006) $25,000.00
Developing a method to measure subunit-subunit interactions inside of a cell
11. Intramural Grant Program (2006) $6,800
Investigating the link between aggregation of p53 and tumor formation
National and local organization leadership
2005 - Chair, Institutional Biosafety Committee, NAU
2006 - 2007 Member, CENS Technology and Indirect Costs Committee, NAU
2000 Member, 6th Annual Biophysics and Cell Biology Symposium organizing committee, Purdue University
1994-1995 President, Student Affiliated ACS Organization, University of Wyoming
Reviewer: for the following journals and organizations
Protein Society
Biomacromolecules
Biotech Progress
Memberships:
2001 - Biophysical Society
2004 - Protein Society
1994-1996 American Chemical Society